Introduction: The specific surface area of montmorillonite is 40 times that of conventional powder. Since montmorillonite has such a strong adsorption capacity, will it also absorb nutrients in the intestinal tract, such as vitamins, and have side effects? However, in a study of the effects of montmorillonite adsorption on nutrients, no adverse effects were found when montmorillonite was added to 25% of the diet by weight, and only when the amount was increased to 50% of the diet did any adverse effects occur.

Objective: To study the adsorption of montmorillonite for water-soluble vitamin B1, B2, B6, B12 C niacinamide

Methods: The concentration of water-soluble vitamins before and after montmorillonite was determined by ultraviolet spectrophotometry or iodimetry, and the adsorption rate was calculated. The adsorption properties of montmorillonite for water-soluble vitamins and its release properties in artificial intestinal fluid were studied. Results: Under experimental conditions, montmorillonite had significant adsorption of vitamin B2 in artificial gastric juice, montmorillonite had significant adsorption of vitamin B1, B2, B6, B12 nicotinamide, but not of vitamin C. The adsorption was in accordance with the Langmuir isothermal adsorption formula, the saturated adsorption volume was г M =76 4mg 'g-1. Adsorbed vitamin B1, B6, B12 nicotinamide was released intact in artificial intestinal fluid. Conclusion: Montmorillonite adsorbs vitamin B1, B6B12 nicotinamide in the stomach obviously, and releases the whole vitamin in the small intestine, so montmorillonite has no effect on the absorption of vitamin B1, B6B12 nicotinamide and even has the release retarding effect, but montmorillonite does not have the absorption and release retarding effect on vitamin C.

Keywords montmorillonite water-soluble vitamin adsorption release

Montmorillonite is an insoluble silicaluminate mineral material with multilayer structure composed of double tetrahedral silicon oxide and octahedral alumina, with an external aperture of 200~800 Nm, this special structure makes it has larger than the surface area and the characteristics of non-average charge dispersion, so it has a strong covering ability, high positioning ability and strong adsorption ability. Montmorillonite can cover the digestive tract, bind with mucous protein, so as to strengthen the mucous barrier, at the same time, the virus of the digestive tract Pathogens and toxins produce strong selective adsorption to avoid the invasion of digestive tract [1] To investigate the long-term or repeated application of montmorillonite, whether may attack the corresponding vitamin deficiency, we use several kinds of water-soluble vitamins as experimental object, through the determination of commonly used dose vitamin liquid medicine to control the content of montmorillonite after and fluid analogy, calculating the adsorption rate and investigation of montmorillonite in vitro adsorption of water-soluble vitamins, Furthermore, the adsorption characteristics of montmorillonite in artificial gastric juice and the dissolution experiment in artificial intestinal juice were discussed, and the influence of montmorillonite on the absorption of water-soluble vitamins was stopped.

Instrument experimental drug

1.1 the instrument

751G- UV-visible spectrophotometer; Electronic analytical balance; Tkh-type liquid rapid mixer (Jiangxi Medical Equipment Stone); Automatic balancing centrifuge; Rc-3b drug dissolution apparatus

1.2 Experimental drugs

Montmorillonite (Inner Mongolia Hezhengmei Biotechnology Co., LTD. Purification products, montmorillonite =96%,Si02 56.32%,AL203 15.28%); Vitamin B1 B2 B6 Vitamin C B12 nicotinamide as medicinal raw materials (in accordance with the Chinese Pharmacopoeia 2000 edition of the second part of the ingot); Dilute hydrochloric acid, sodium thiosulfate for analysis of pure

2. experimental methods and results

2.1 Rules for the content of liquid before montmorillonite participation

Finely weigh vitamin B1 B6 25mg in four parts, nicotinamide 60 mg in vitamin C 200 mg in four parts, with four samples of each vitamin taken in equal quantities. Dilute B12 to 015 mg ml-1 and measure out 1.0 mL of it in a 100ml flask for 4 portions. Take one copy of each of the above-mentioned fine weighing vitamins and put them into 100 ml bottles respectively. According to the pharmacopoeia method, vitamin C was determined by iodimetry, and other vitamins were determined by ultraviolet spectrophotometry

2.2 Determination of vitamin content after montmorillonite in water and artificial gastric juice Take one of the vitamins finely weighed in 2.1, place them in 100 ml flask, add water to the scale, and shake on the mixer to make the vitamins fully dissolved. Add 1.5625g of montmorillonite (equivalent to 115 g of montmorillonite), add water to constant volume and take another 1.5625g of montmorillonite powder into 100ml measuring bottle, add water to scale as blank control. Shake it at room temperature for 5 min, place it for 30 min, take the supernatant at 315 RPM/separate center, dilute it according to 2.1 corresponding drug determination method and determine the content. Take another vitamin of fine weighing in 2.1, and determine the vitamin content of the above vitamins after montmorillonite in artificial gastric juice with the same method

The adsorption of montmorillonite for vitamin B2 was carefully weighed 10 mg in two equal parts, placed in 100 mL bottles, respectively, and dissolved according to the pharmacopoeia method. The content of one part was determined according to the pharmacopoeia method [3], and the other part was added according to 2.2 method, and the supernatant was taken to determine the content by the same method

Calculation of adsorption rate After the absorption of each drug measured minus the corresponding blank control, calculate the drug concentration before and after adsorption, adsorption rate =(1- drug concentration after adsorption solution/drug concentration before adsorption)100%. As can be seen from Table 1, vitamin B1, B6B12 under artificial gastric juice or water, vitamin B2 under experimental conditions, compared with the determination results of control liquid, has a frequent and obvious drop in absorption, montmorillonite has significant adsorption of the above vitamins. Vitamin C under the condition of water and artificial gastric juice, the titration results with controlled liquid, niacinamide montmorillonite on its adsorption in aqueous solution, and there was no difference in the controlled liquid absorbance, montmorillonite on its adsorption, with the literature [4] differences in artificial gastric juice, the determination results compared with control fluid, absorbance has very obvious descent

2.3 Release of adsorbed vitamins in artificial intestinal fluid

The above vitamins were weighed and placed in a 50 mL flask, and artificial gastric juice was added for about 48 ml to make it completely dissolved. 1.5625 g montmorillonite was added, artificial gastric juice was brought to the scale, the mixer was oscillated for 5 min, and the mixture was left to stand for 30 min before centrifugation. The vitamin content in the solution when the adsorption reached equilibrium was determined according to method 2.1, and the apparent adsorption amount was calculated Discard the supernatant, use the slurry method, transfer the precipitate completely to the dissolution cup, take 900 mL artificial intestinal fluid, control the temperature at 371, rotate speed at 100 RPM, extract 10 mL solution at 5 10 30 minutes, and every 30 minutes thereafter, calculate the vitamin content and cumulative release amount in the solution at the time of sampling according to method 2.1, and supplement at the same time After 30 minutes, the vitamin concentration in the dissolved solution did not increase. According to the apparent adsorption amount, the absorbed vitamins were released completely in the artificial intestinal solution

Discuss

Montmorillonite aluminum oxide octahedral layer and silicon oxygen tetrahedral layer alternate arrangement structure, can be seen as the octahedral layer is sandwiched between two tetrahedral layers, due to the octahedral layer in the local Al3+ Si2+ replaced with negative charge, so there are often Na+ K+ Ca2+ H+ plasma between montmorillonite layer, these ions can be with other positively charged ions (such as Cu2+ Zn2+ Fe3+ quaternary ammonium cations, etc.) stop ion exchange. Therefore, due to its special internal structure, montmorillonite has unsaturated negative charge and strong cation exchange ability, which is manifested as cation adsorption [5] vitamin B1 Vitamin B6 is thiamine hydrochloride and pyridoxine hydrochloride, which are cations. Therefore, montmorillonite has significant adsorption on vitamin B6 either in water or in artificial gastric juice Vitamin B2 is amphoteric compound, soluble in acid and base, hardly soluble in water. Under experimental conditions (pH about 4.5), the lone pair electron of the tertiary nitrogen atom of isorozine 10 absorbs protons and separates from it, showing cationic characteristics, so it has certain adsorption to montmorillonite. Vitamin B12 is the cobalt inner complex salt of imidazolinic acid and coraline ring, cobalt atoms have positive charge, so montmorillonite adsorbed to it under neutral and acidic conditions. Vitamin C molecules in the structure of the enol lactone, strong acid, zeta potential is relatively weak, and not be montmorillonite adsorption nicotinamide to neutral compounds, therefore not be adsorption in aqueous solution nitrogen atom in the pyridine ring in artificial gastric juice for the separation of electrons and protons, show the weak alkaline, as cationic characteristics, so the montmorillonite on the adsorption The amount of montmorillonite adsorption organic cation and montmorillonite ion exchange ability and the variety of ions contained, also related to the varieties of organic cations, montmorillonite and organic cation exchange reaction is equivalent exchange, so it has saturated adsorption characteristics of vitamins and dissolution characteristics of the research indicated; The adsorption of vitamins in intestinal fluid conditions can be released in a short time, because the absorption of vitamins is mainly in the small intestine, so montmorillonite adsorption of vitamins in the body has no effect on its absorption, and has a slow release effect, but montmorillonite does not exist on the absorption and release of vitamin C